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The method is that showed by Mrs Lauretta Pasquini, Mr Giovanni Lo Bue,
Mr Gianluigi Gregari and by Mr Giovanni Magiorotto at Spoleto study-congress
on truffle-growing.
Summary of the method. For further details consult the study-congress
relation of Spoleto, may 1999, or contact Mr Rogier Pierre (see the above-written
address).
"Take a plastic bottle, cut it lengthwise into two equal parts. In
a tree to mycorrhiza look for a root of about a pencil thick. Cut it of
the same bottle length and deprive, what remain stuck to the tree, of
all the secondary roots. Fill in the half bottle with a mixture of sterile
earth and vermiculite of the same quantity, mingle it with some spores
of a real melanosporum truffle and humidify the whole a little. Place
the root on this half bottle. Fill in the other half with the same mixture.
Join together the two halves and bind them with a rope or some rubber
bands. Place the whole in a hole of 20 to 30 centimetres deep and cover
it, at least, by 15 centimetre of earth. Water to make the whole more
solid. Wait about eight months and then take the bottle out of the hole.
Spread the secondary roots with earth, water them and wait."
My friends and I have transplanted 35 bottles and, in order to point out
existing mycorrhizae, we carefully examined the secondary roots, dissected
on the root, on the microscope. So I was able to discover some mycorrhizae
similar to those of a winter truffle, Melanosporum, AD, Basidiomycins,
Mesentericum, Aestivum, graniform Cenococcum and, of course, some undefined
truffles. All of these observations have been surveyed and arranged in
bottles.
I havent taken my bottles out of the hole yet but I will do it very
soon to examine the mycorrhization of each and, hoping that it will be
good, I will wait for some results. I think Ill do those experiments
again this year under the same proportions. Mr Aimé Richaud de
St Michel, of the Low Alps Observatory, has also tried those methods and,
in 1994, he transplanted two bottles: one on an oak-tree which, until
now, hasnt given any results yet and one on a lime-tree which has
given him the following results:
1st harvest 1996/1997 = 2 kilos of melanosporum
2nd harvest 1997/1998 = 4,5 kilos
3rd harvest 1998/1999 = 7 kilos
4th harvest from 1999 to 11-02-2000 = 5 kilos
It must be said that the quality of these melanosporum truffles is perfect.
The result is certainly an excellent one but this experience deserves
to be brought forward and if there are some people who wish to do it I
will be at their disposal for further details. I would also love to know
their bad or good results.
As for as I am concerned when I take the bottles out of the hole I will
make a new report on the evolution and on the possible results of this
experiment, which I will communicate you on a simple request.
Dear truffle-growers, I want you to pluck up courage for this experiment
and wish you good results as those of our friend Mr Richard.
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