Experiment of mycorrhization on some-year-old trees.

The method is that showed by Mrs Lauretta Pasquini, Mr Giovanni Lo Bue, Mr Gianluigi Gregari and by Mr Giovanni Magiorotto at Spoleto study-congress on truffle-growing.
Summary of the method. For further details consult the study-congress relation of Spoleto, may 1999, or contact Mr Rogier Pierre (see the above-written address).
"Take a plastic bottle, cut it lengthwise into two equal parts. In a tree to mycorrhiza look for a root of about a pencil thick. Cut it of the same bottle length and deprive, what remain stuck to the tree, of all the secondary roots. Fill in the half bottle with a mixture of sterile earth and vermiculite of the same quantity, mingle it with some spores of a real melanosporum truffle and humidify the whole a little. Place the root on this half bottle. Fill in the other half with the same mixture. Join together the two halves and bind them with a rope or some rubber bands. Place the whole in a hole of 20 to 30 centimetres deep and cover it, at least, by 15 centimetre of earth. Water to make the whole more solid. Wait about eight months and then take the bottle out of the hole. Spread the secondary roots with earth, water them and wait."
My friends and I have transplanted 35 bottles and, in order to point out existing mycorrhizae, we carefully examined the secondary roots, dissected on the root, on the microscope. So I was able to discover some mycorrhizae similar to those of a winter truffle, Melanosporum, AD, Basidiomycins, Mesentericum, Aestivum, graniform Cenococcum and, of course, some undefined truffles. All of these observations have been surveyed and arranged in bottles.
I haven’t taken my bottles out of the hole yet but I will do it very soon to examine the mycorrhization of each and, hoping that it will be good, I will wait for some results. I think I’ll do those experiments again this year under the same proportions. Mr Aimé Richaud de St Michel, of the Low Alps Observatory, has also tried those methods and, in 1994, he transplanted two bottles: one on an oak-tree which, until now, hasn’t given any results yet and one on a lime-tree which has given him the following results:
1st harvest 1996/1997 = 2 kilos of melanosporum
2nd harvest 1997/1998 = 4,5 kilos
3rd harvest 1998/1999 = 7 kilos
4th harvest from 1999 to 11-02-2000 = 5 kilos
It must be said that the quality of these melanosporum truffles is perfect. The result is certainly an excellent one but this experience deserves to be brought forward and if there are some people who wish to do it I will be at their disposal for further details. I would also love to know their bad or good results.
As for as I am concerned when I take the bottles out of the hole I will make a new report on the evolution and on the possible results of this experiment, which I will communicate you on a simple request.
Dear truffle-growers, I want you to pluck up courage for this experiment and wish you good results as those of our friend Mr Richard.

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